ADRB3 antibody (AA 179-250)

Catalog No. ABIN7600307

This anti-ADRB3 antibody is a Rabbit Polyclonal antibody detecting ADRB3 in WB, ELISA, IF and ICC. Suitable for Human, Rat and Mouse.

Quick Overview for ADRB3 antibody (AA 179-250) (ABIN7600307)

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADRB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ADRB3 Antibody

Binding Specificity: AA 179-250

Purpose: Anti-beta 3 Adrenergic Receptor/ADRB3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-beta 3 Adrenergic Receptor/ADRB3 Antibody Picoband® (ABIN7600307). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human beta 3 Adrenergic Receptor/ADRB3 recombinant protein (Position: R179-S250).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bachman, E. S., Dhillon, H., Zhang, C.-Y., Cinti, S., Bianco, A. C., Kobilka, B. K., Lowell, B. B. Beta-AR signaling required for diet-induced thermogenesis and obesity resistance. Science 297: 843-845, 2002. 2. Buettner, R., Schaffler, A., Arndt, H., Rogler, G., Nusser, J., Zietz, B., Enger, I., Hugl, S., Cuk, A., Scholmerich, J., Palitzsch, K.-D. The trp64arg polymorphism of the beta-3-adrenergic receptor gene is not associated with obesity or type 2 diabetes mellitus in a large population-based Caucasian cohort. J. Clin. Endocr. Metab. 83: 2892-2897, 1998. 3. Cagliani, R., Fumagalli, M., Pozzoli, U., Riva, S., Comi, G. P., Torri, F., Macciardi, F., Bresolin, N., Sironi, M. Diverse evolutionary histories for beta-adrenoreceptor genes in humans. Am. J. Hum. Genet. 85: 64-75, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.01 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADRB3

Target: ADRB3 (Adrenergic, beta-3-, Receptor (ADRB3))

Alternative Name: ADRB3

Background:

Synonyms: Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor, ADRB3, ADRB3R, B3AR

Tissue Specificity: Expressed mainly in adipose tissues.

Background: The beta-3 adrenergic receptor (β3 adrenoreceptor), also known as ADRB3, is a beta-adrenergic receptor, and also denotes the human gene encoding it. It is mapped to 8p11.23. The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.

Molecular Weight: 44 kDa

Gene ID: 155

UniProt: P13945

Pathways: cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, Brown Fat Cell Differentiation